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1.3.3: Instruments of Microscopy - Biology

Learning ObjectivesIdentify and describe the parts of a brightfield microscopeCalculate total magnification for a compound microscopeDescribe the distinguishing features and typical uses for various types of light microscopes, electron microscopes, and scanning probe microscopesThe early pioneers of microscopy opened a window into the invisible world of microorganisms.
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PCR in different cell line

I developed a PCR cycle to amplify a 5.5 kb genomic DNA region in HEK cell line. When I tried to use the same protocol in A375 cell line I had no results but only smear. Can you help me? Thanks in
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